# Strain collections

**URL:** https://community.alliancegenome.org/c/model-organism-yeast/strain-collections/242.md

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## [About the Strain collections category](https://community.alliancegenome.org/t/about-the-strain-collections-category/7335)

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**Author:** [@stacia](https://community.alliancegenome.org/u/stacia)\
**Replies:** 0

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A place for researchers to discuss yeast strain collections. Do you have collections available to share? Are you looking for strains? Do you have questions about any strains that you’re using in the lab? Have you found…

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## [Comprehensive overview of yeast libraries](https://community.alliancegenome.org/t/comprehensive-overview-of-yeast-libraries/8565)

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**Author:** [@edith](https://community.alliancegenome.org/u/edith)\
**Replies:** 0\
**Last updated:** [November 25, 2025, 6:35pm UTC](https://community.alliancegenome.org/t/comprehensive-overview-of-yeast-libraries/8565 "2025-11-25T18:35:08Z")

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https://febs.onlinelibrary.wiley.com/doi/10.1111/febs.70325 Baruch et al. have compiled and published a paper that describes a comprehensive list of genome-wide collections of Saccharomyces cerevisiae. They have grouped…

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## [Where to find Yeast-GFP clones](https://community.alliancegenome.org/t/where-to-find-yeast-gfp-clones/7340)

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**Author:** [@stacia](https://community.alliancegenome.org/u/stacia)\
**Replies:** 0\
**Last updated:** [November 16, 2023, 7:34pm UTC](https://community.alliancegenome.org/t/where-to-find-yeast-gfp-clones/7340 "2023-11-16T19:34:49Z")

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We get this question at SGD a lot: “Where can we get the yeast-GFP clones?” The only thing that we can find about #GFP strains post-ThermoFisher/Invitrogen is on Maya Schuldiner’s lab page: https://mayaschuldiner.wixsi…

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## [New yeast library: H2O2 sensor HyPer7 fused to C-terminus of all ORFs](https://community.alliancegenome.org/t/new-yeast-library-h2o2-sensor-hyper7-fused-to-c-terminus-of-all-orfs/7364)

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**Author:** [@stacia](https://community.alliancegenome.org/u/stacia)\
**Replies:** 0\
**Last updated:** [November 29, 2023, 5:03pm UTC](https://community.alliancegenome.org/t/new-yeast-library-h2o2-sensor-hyper7-fused-to-c-terminus-of-all-orfs/7364 "2023-11-29T17:03:40Z")

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https://www.pnas.org/doi/pdf/10.1073/pnas.2314043120 Kritsiligkou et al. have developed a yeast library in which every protein is equipped with its own proximal H2O2 probe. Use of this library reveals redox differenc…

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## [Yeast KO strain validation](https://community.alliancegenome.org/t/yeast-ko-strain-validation/7349)

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**Author:** [@ffontanesi](https://community.alliancegenome.org/u/ffontanesi)\
**Replies:** 6\
**Last updated:** [November 27, 2023, 4:13pm UTC](https://community.alliancegenome.org/t/yeast-ko-strain-validation/7349 "2023-11-27T16:13:12Z")

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In our experience a significant number of Saccharomyces cerevisiae knock-out strains in the BY4741 (MAT a) and BY4742 (MAT alpha) genetic background still carry a copy of the wild-type gene. We test all strains by PCR us…

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## [Commonly used auxotrophic markers](https://community.alliancegenome.org/t/commonly-used-auxotrophic-markers/7338)

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**Author:** [@stacia](https://community.alliancegenome.org/u/stacia)\
**Replies:** 0\
**Last updated:** [November 16, 2023, 6:53pm UTC](https://community.alliancegenome.org/t/commonly-used-auxotrophic-markers/7338 "2023-11-16T18:53:36Z")

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Allele Deleted ORF? Reverts? Notes Molecular Descriptiona Reference ade1-14 no yes red colonies TGG-to-TGA nonsense change at codon 244; GGA-to-GAA missense change at codon 185 Nakayashiki et al. 2001 ade1-101 n…

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## [Yeast Stock Centers](https://community.alliancegenome.org/t/yeast-stock-centers/7337)

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**Author:** [@stacia](https://community.alliancegenome.org/u/stacia)\
**Replies:** 0\
**Last updated:** [November 16, 2023, 6:46pm UTC](https://community.alliancegenome.org/t/yeast-stock-centers/7337 "2023-11-16T18:46:32Z")

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American Type Culture Collection (ATCC) maintains yeast stocks and clones. EUROSCARF, the EUROpean Saccharomyces Cerevisiae ARchive for Functional analysis, maintains a collection of systematic deletion strains sear…

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## [Strain Collections](https://community.alliancegenome.org/t/strain-collections/7336)

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**Author:** [@stacia](https://community.alliancegenome.org/u/stacia)\
**Replies:** 0\
**Last updated:** [November 16, 2023, 6:44pm UTC](https://community.alliancegenome.org/t/strain-collections/7336 "2023-11-16T18:44:43Z")

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Yeast-GFP Clone Collection from Dr. Erin O’Shea and Dr. Jonathan Weissman at UCSF, consisting of C-terminal tagged open reading frames (ORFs). These may be available from the Schuldiner Lab SWAT. Yeast GST-Tagged Co…
