20 degree developmental timeline for C. elegans

Model Organism: Worms Scientific Discussion
1

I am looking for a developmental timeline following feeding of newly hatched L1 larvae (hatched o.n. in M9) at 20 degrees. There is a timeline in the old C. elegans book at 25 degrees, but I have not found one at 20. Can anybody steer me to a reference? John Lenard (lenard@umdnj.edu).

2

Hi John,

Michael Koelle’s has what you need:

http://info.med.yale.edu/mbb/koelle/protocols/protocol_timing-staging.html

Cheers,
Cha

3

For a more detailed timeline, the postembryonic lineage charts from Sulston & Horvitz (Devel. Biol 1977) are the standard for 20C. You can find the paper online from the WormAtlas site. -Paul