# A family of transcripts in F10C2.6

**URL:** <https://community.alliancegenome.org/t/a-family-of-transcripts-in-f10c2-6/3130>\
**Category:** WormBase\
**Created:** [January 16, 2008, 6:57am UTC](https://community.alliancegenome.org/t/a-family-of-transcripts-in-f10c2-6/3130 "2008-01-16T06:57:29Z")\
**Posts on this page:** 1\
**Page:** 1

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**Author:** ![gw3](https://community.alliancegenome.org/letter_avatar_proxy/v4/letter/g/b4bc9f/32.png) [@gw3](https://community.alliancegenome.org/u/gw3)\
**Post date:** [January 16, 2008, 6:57am UTC](https://community.alliancegenome.org/t/a-family-of-transcripts-in-f10c2-6/3130/1 "2008-01-16T06:57:29Z")

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I just thought you might enjoy this one…  
🙂

The 3’ splice site of the penultimate intron (intron 5) does not have a strongly scoring splice site and the EST alignments show that splicing occurs at several sites within a region of about 50 bases. This results in a family of transcripts all differing slightly as they splice into different positions around the end of this intron.

There is mass-spectroscopy evidence that this gene produces a protein product.  
There is some RNAi evidence of a phenotype (embryonic lethal, maternal sterile).

It looks as if there has been an integration of a transposon-like element in this intron which has distrupted the original 3’ splice site and now the transcription mechanism is producing a family of transcripts splicing to several different sites over a region of about 50 bases. Some of these transcripts are then viable enough to avoid being destroyed by NMD and to make a protein detectable by mass-spec.

I would expect to see strong selection at this locus for variants with any sort of in-frame splice site motif.

Gary