# August updates to the FlyBase Papers with Technical Advances page **URL:** \ **Category:** Technical advances\ **Created:** [September 1, 2025, 9:33pm UTC](https://community.alliancegenome.org/t/august-updates-to-the-flybase-papers-with-technical-advances-page/8496 "2025-09-01T21:33:56Z")\ **Posts on this page:** 1\ **Page:** 1
**Author:** ![sian](https://community.alliancegenome.org/user_avatar/community.alliancegenome.org/sian/32/907_2.png) [@sian](https://community.alliancegenome.org/u/sian)\ **Post date:** [September 1, 2025, 9:33pm UTC](https://community.alliancegenome.org/t/august-updates-to-the-flybase-papers-with-technical-advances-page/8496/1 "2025-09-01T21:33:56Z")
It’s August Technical Advances update time! See the newly added papers here [FlyBase:Papers with technical advances - FlyBase Wiki](https://wiki.flybase.org/wiki/FlyBase:Papers_with_technical_advances#Aug_2025). Don’t forget to flag when you Fast-Track Your Paper if you want your paper with a new technique, reagent, or resource featured next month. Generation of six4 -nlsRFP: a red somatic gonadal nuclear marker for live imaging Drosophila gonadogenesis. (MicroPubl Biol) Reagent to detect somatic gonadal cell nuclei with RFP during embryogenesis > **[Generation of six4 -nlsRFP: a red somatic gonadal nuclear marker for live...](https://pubmed.ncbi.nlm.nih.gov/40672113/)** > > Intrinsically fluorescent tissue labels are valuable to live image cell behaviors during development. Fluorescent markers of Drosophila gonadal cells have elucidated dynamic cellular interactions that shaped our conception of cell biology. Many green... Insulator BEAF32 regulates expression of tissue-specific genes and piRNA source loci in Drosophila ovaries. (Epigenetics Chromatin) We generated a heatmap with hierarchical clustering of the differentially expressed Drosophila gene groups which we called Anatomy Gene expression in Fly (AnGeFly) and created a web-based application [[https://akob.shinyapps.io/angefly/](https://akob.shinyapps.io/angefly/)] > **[Insulator BEAF32 regulates expression of tissue-specific genes and piRNA...](https://pubmed.ncbi.nlm.nih.gov/40717082/)** > > Our findings suggest that BEAF32 coordinates multiple transcriptional regulatory functions important for Drosophila oogenesis. BEAF32 represses the ectopic expression of developmental and tissue-specific genes in the ovaries. BEAF32 regulates polo... A deep learning model for accurate segmentation of the Drosophila melanogaster brain from Micro-CT imaging. (Dev. Biol.) We generated a deep learning AI model for automated segmentation of the adult fly brain from microcomputed tomography imaging. This model is freely available and can be adapted to individual user needs. > **[A deep learning model for accurate segmentation of the Drosophila...](https://pubmed.ncbi.nlm.nih.gov/40447251/)** > > The use of microcomputed tomography (Micro-CT) for imaging biological samples has burgeoned in the past decade, due to increased access to scanning platforms, ease of operation, and the advance of software platforms that enable accurate... Deep learning-based high-resolution time inference for deciphering dynamic gene regulation from fixed embryos. (Nat. Commun.) We developed a deep learning algorithm to infer the developmental time from fixed Drosophila embryo images (nc11-early nc14) at 1-minute resolution, without the need for live imaging. > **[Deep learning-based high-resolution time inference for deciphering dynamic...](https://pubmed.ncbi.nlm.nih.gov/40670357/)** > > Embryo development is driven by the spatiotemporal dynamics of complex gene regulatory networks. Uncovering these dynamics requires simultaneous tracking of multiple fluctuating molecular species over time, which exceeds the capabilities of... Growth phase diets diminish histone acetyltransferase Gcn5 function and shorten lifespan of Drosophila males. (EMBO Rep.) live yeast-fly assay: We imposed dietary interventions throughout the entire larval stages using the KO collection of yeast, and then quantified the lifespans of eclosed adults on the common standard food to assess how the larval diets affect the adult health. > **[Growth phase diets diminish histone acetyltransferase Gcn5 function and...](https://pubmed.ncbi.nlm.nih.gov/40640422/)** > > The nutritional environment in early life, referred to as the nutrition history, exerts far-reaching health effects beyond the developmental stage. Here, with Drosophila melanogaster as a model, we fed larvae on diets consisting of a variety of yeast... Nonmuscle myosin II regulates presynaptic actin and neuronal mechanobiology in Drosophila. (J. Cell Biol.) QUAS-mScarlet-I::Act5C, QUAS-Tm1-A::mNeonGreen, QUAS-Tm1-L::mNeonGreen, QUAS-mNeonGreen::GMA > **[Nonmuscle myosin II regulates presynaptic actin and neuronal mechanobiology...](https://pubmed.ncbi.nlm.nih.gov/40644605/)** > > Neuromuscular junctions (NMJs) are evolutionarily ancient, specialized contacts between neurons and muscles. They experience lifelong strain, yet the mechanism preserving their integrity under mechanical load remains unclear. Here, we identify a... Evolution of a novel left-right asymmetry in organ size by co-option of a tissue rotation process. (Development) Membrane EYFP marker pB-act5C::DE-Cad-EYFP\_2: shg 5’UTR, first two cadherin repeats, trans-membrane domain, part of the cytoplasmic domain without the beta-Catenin binding domain, inserted into pBAC-ECFP-15xQUAS\_TATA-mcd8-GFP-SV40 (Addgene 104878) with integration marker 3xP3 DsRed. > **[Evolution of a novel left-right asymmetry in organ size by co-option of a...](https://pubmed.ncbi.nlm.nih.gov/40600793/)** > > Left-right asymmetries have repeatedly evolved in diverse animals and affect the position, shape or size of specific organs. How novel left-right asymmetries arise remains unknown. Here, we examined Drosophila pachea, where males have evolved unique... High-throughput measurement of Drosophila feeding behavior. (Cell Rep Methods) FlyPlate-BCA provides a high-throughput, accurate method to quantify food intake in Drosophila via BCA protein assay, improving sensitivity and reducing sample requirements for diet and aging research. > **[High-throughput measurement of Drosophila feeding behavior - PubMed](https://pubmed.ncbi.nlm.nih.gov/40683246/)** > > Accurate measurement of Drosophila feeding is vital for metabolic and aging studies, but current assays lack the throughput and sensitivity needed for large-scale screens. We introduce FlyPlate-BCA, a 96-well platform that combines automated... Dying occurs as a defined molecular progression in Drosophila rather than as nonspecific physiological collapse. (iScience) Pseudotime analysis of single-fly transcriptomic data to overcome population heterogeneity in analysis of a phenotype > **[Dying occurs as a defined molecular progression in Drosophila rather than as...](https://pubmed.ncbi.nlm.nih.gov/40755455/)** > > While we know much about aging, dying remains mysterious. Here, we show that old-age death in Drosophila is an orderly process that follows, but is distinguishable from, aging itself. We apply pseudotime trajectory analysis to transcriptomic data... Temperature-dependent modulation of light-induced circadian responses in Drosophila melanogaster. (EMBO J.) Monitoring the calcium activity of neurons in real-time under a two-photon microscopy in live Drosophila, which can be coupled with optogenetics. > **[Temperature-dependent modulation of light-induced circadian responses in...](https://pubmed.ncbi.nlm.nih.gov/40588550/)** > > Animals entrain their circadian rhythms to multiple external signals, such as light and temperature, which are integrated in master clock neurons to adjust circadian phases. However, the precise mechanisms underlying this process remain unclear.... Endogenous OptoRhoGEFs reveal biophysical principles of epithelial tissue furrowing. (Nat. Commun.) endogenously-tagged optogenetic RhoGEFs > **[Endogenous OptoRhoGEFs reveal biophysical principles of epithelial tissue...](https://pubmed.ncbi.nlm.nih.gov/40825942/)** > > During development, epithelia function as malleable sheets that undergo extensive remodeling to shape developing embryos. Optogenetic control of Rho signaling provides an avenue to investigate mechanisms of epithelial morphogenesis, but transgenic...