balancer near dpy-5

Model Organism: Worms Methods & Reagents
1

hi All:

Does anyone have a GFP or mCherry integrant very close to dpy-5 on LG I that is easily scorable with a flourescence dissecting scope? I need to balance a sterile mutant and a visible balancer would be ideal.

thank you!
AndyG

2

Unless you’ve got some reason to avoid it (the fact that it also balances part of LGIII, for example), the balancer you want is probably hT2 [bli-4(e937) let(q782) qIs48]: balances dpy-5 (balances LGI from the left end to ~13, in fact), linked recessive lethal, strong GFP expression. I believe this is the standard strain.
There is a graphical map of balancers in the Wormbook chapter on the subject (Edgley, Bailey, Riddle, and Rose). The Genetic Map hasn’t been updated in a while (so far as I know the 2003 version is the last one compiled in this form) but it has an excellent and still-useful table of balancers then available.

3

thanks Hillel,

we have been using hT2 but it does break down (or whatever). It is a much better balancer for III; i’m guessing the GFP is on the part of the translocation that is III. When we balance things on LG I near dpy-5, we occasionally find non-green animals that do not display our phenotype. We have yet to see this for anything we balance on III though. I was hoping someone had a nice bright GFP that they knew mapped near dpy-5. With all the transgenic insertions, I assume some of us have done some mapping to know where our insertions are.

Thanks though. Hopefully someone else will have a good GFP or mCherry marker.

cheers,
Andy

4

Hi,

You can try to use okIs59, a bright pharyngeal GFP mapped between dpy-5 & unc-13. It’s available at the CGC.

https://cgcdb.msi.umn.edu/strain.php?id=13626

http://www.zoology.ubc.ca/~dgmweb/research1_strains2.htm

~Mallory~