I am trying to detect the expression of a transgene protein in the neuron. I used Bouin’s fix solution and BTB (Boric acid, triton x 100,mercaptoethanol) to fix and permeabilize the worms, I froze and thawed 3 times with dry ice and ethanol,
I could not get the adult worms stained, only the very young worms show positive.
Is it because that the adult worms have a thick cuticle? I am considering using collagenase, will collagenase treatment help?
Thanks in advance!