cDNA

Dear all,

I had posted the lengthy question below, but really woundered, how about just cDNA? Does anyone have or know of a really cheep place (below 2000$)?

Stephan

Origional post----

-I’m new to this web site. I’m a structural biologist. I am interested in some proteins with overlap between species. I may be moving -to Brazil in the near future, and am trying to acquire several things to get started.

-My main problem is cheep or free cDNA for c.elegans, and then I am also looking for protocols in large scale worm growth (normal -conditions, normal or over expressed protein clones, etc) with the aim of protein purification. In a similar manner, I am interested in -nuclear extraction protocols (or leftovers if anyone wants to give them up), as human nuclear extracts are pricy and hard to get -enough for structural work if done in the lab. Basically, I would like to have 200 ml at 2 mg/ml protein conc. nuclear extract in the end, -from worm culture. I need however, to find out feasibility of such things, as I only know worm protocols from several courses, but -have not used them much directly (other than a protein or two in e.coli). Any suggestions or comments (or supplies) are -appreciated, and I would gladly give the Brazilian lab address if anyone needs. I most likely will post other questions and searches -for material here in the future.

-mit freundlichen Grüssen,

-Stephan Watkins

You can get EST clones for many genes from Yuji Kohara for free.

These are all the cDNAs you see in wormbase with a yk prefix. See:

http://www.wormbase.org/wiki/index.php/FAQs#Whom_could_I_contact_about_getting_a_cDNA_clone.3F

Freddie

Thanks.

I think I figured out how things work in ordering. Whatever happened to whole organism cDNA soup for PCRing almost anything. I still have a hard time sorting through the free YK clones?

Stephan