I have a basic question about transmission of extrachromosomal array after injection. So, I have been using the pRF4 plasmid together with PCR of my gene of interest to inject into wild type worms. As expected, I get both roller as well as non-roller worms. I read somewhere that the marker plasmid and DNA may not always cosegregate. Has anyone had any experience like this? I wanted to be sure that the transgenics I analyse are actually carrying my DNA of interest besides the rolle/marker plasmid.