Crossing strains with mutations on same chromosome

Model Organism: Worms Methods & Reagents
1

I want to cross two strains with different mutations on two genes to obtain a strain with both mutations.
Unfortunately the genes are on the same chromosome.

Somebody has a recommendations how to do this?

Thanks for help.

2

Conceptually, it’s an easy experiment. Mate the strains containing the desired mutations and recover recombinant lines. In practice, how easy or difficult it will be depends on a number of factors.

  1. How closely linked are the two mutations?
  2. Do the mutations have phenotypes that are easily scored?
  3. If not, are there molecular tools (e.g., Snip-SNPs) for screening the mutations?
  4. Does either mutation affect progeny production (mating efficiency/lethality/sterility)?
  5. Is either (or both) mutation(s) linked to a morphological marker(s)?

Absent these details, it’s difficult to propose a plan of attack.

-harold

3

I found this helpful when I was in a similar situation.

http://www.wormbook.org/chapters/www_makingcompdmutants.2/makingcompdmutants.html

4

Thanks ‘snug’ and Harold.
I will go for the standard crossing and hope for some fortunate crossing over.