CTX (or other?) buffer for NaCl starvation associative learning

Model Organism: Worms Methods & Reagents
1

Hello,

I am about to do an NaCl starvation associative learning experiment after several months of butanone-food associative learning experiments. Most protocols for NaCl-starvation assays call for the NaCl to be diluted in CTX buffer, which I can not find a recipe for.

Does anyone either 1) have a CTX recipe, or 2) have another NaCl diluent suggestions?

Thanks!

2

anw09,

details are in the wormbook;

http://www.wormbook.org/chapters/www_behavior/behavior.html

“During a 15 min wash, the well-fed, young adult nematodes are washed three times with CTX buffer (5 mM KH2PO4/K2HPO4 pH 6, 1 mM CaCl2 and 1 mM MgSO4)”

Steve