CTX (or other?) buffer for NaCl starvation associative learning


I am about to do an NaCl starvation associative learning experiment after several months of butanone-food associative learning experiments. Most protocols for NaCl-starvation assays call for the NaCl to be diluted in CTX buffer, which I can not find a recipe for.

Does anyone either 1) have a CTX recipe, or 2) have another NaCl diluent suggestions?



details are in the wormbook;


“During a 15 min wash, the well-fed, young adult nematodes are washed three times with CTX buffer (5 mM KH2PO4/K2HPO4 pH 6, 1 mM CaCl2 and 1 mM MgSO4)”