Does anyone have experience using cycloheximide to inhibit protein translation in whole worms? I’d like to use it for protein stability assays.
Yes, protocols and data for protein stability are in
Zdinak, L.D., Greenberg, I.M., Szewczyk, N.J., Barmada, S.J., Cardamone-Rayner, M. Hartman, J.J. & Jacobson, L.A. Transgene-coded chimeric proteins as reporters of intracellular proteolysis: Starvation-induced proteolysis of a lacZ fusion protein in muscle cells of Caenorhabditis elegans J. Cell. Biochem. 67:143-153 (1997)
Thanks, we’ll try it.