According to this paper, (Culture of embryonic C. elegans cells for electrophysiological and pharmacological analyses), we can detached cell by
repeat pipetting.
Since we use peanut lectin as their base to promote cell adhere and differentiation, how can we detached them rather than repeat pipetting?
Usually, in cell culture, they use acutase or trypsin. But their function optimize in 37 degree.
How about c elegans cells?