I have just started working with C.elegans as the provider of protein to a crystallography project, which obviously demands high amount of protein from C.elegans. While scaling the production of the worms is no problem, we would like to know what is the most efficient way of disrupting the worms without introducing any unnecessary heating etc.
We have looked at the nitrogen bomb technique but before ordering the equipment, we would like to hear if anyone here has any experiences with using the nitrogen bomb technique for disrupting C.elegans??? or if anybody have another way of disrupting the worms please feel free to share.
any information on the matter is appreciated
Thx a lot in advance
p.s. as a newbie in the worm field I found the registrations verification questions quite hard!!