Embryo extracts by Dounce grinder

Hi, all,

I have read the thread on extracting worm proteins with the reference (Chen et al., 2000) on embryo fractionation with the Dounce grinder. We currently don’t have a Dounce grinder in the lab but I was told that there are difference Dounce grinders that have different fits to give final particles of different sizes. ??? Can someone provide a more detailed protocol using the Dounce grinder and a brand name with catalog number of the specific model you use? Thanks a million!