last week I did some FITC staining of different stage eggs and noticed that in addition to the normal outline of the egg shell, prominent bright spots on the surface of different stage eggs.
Some had two, some one and at least one had three. It can’t be an artefact as the positioning seems relatively conserved, so I was wondering whether they were stained polar bodies…but then shouldn’t the egg be effectively impermeable to FITC in the later stages?
At least one of the eggs show at the links below is late stage.
Anyone cast light on what is probably a frequent observation to trained embryologists.
P.S. apologies for the poor quality of the images, we have no DIC and the inverted scope is limited in it’s capacity to resolve bright field. We’re saving up our pennies for a good scope.