# For 2% Agar pads **URL:** \ **Category:** WormBook\ **Created:** [September 15, 2014, 9:43am UTC](https://community.alliancegenome.org/t/for-2-agar-pads/4984 "2014-09-15T09:43:22Z")\ **Posts on this page:** 9\ **Page:** 1
**Author:** ![Okayforum](https://community.alliancegenome.org/letter_avatar_proxy/v4/letter/o/f19dbf/32.png) [@Okayforum](https://community.alliancegenome.org/u/Okayforum)\ **Post date:** [September 15, 2014, 9:43am UTC](https://community.alliancegenome.org/t/for-2-agar-pads/4984/1 "2014-09-15T09:43:22Z")
Good day to the forum members. Please I need help with my experiment on the worms. Am trying to make 2% agar pads that would be used for mounting the worms. I have gotten the protocol from [wormbook.org](http://wormbook.org) and it was mentioned there that 5% agar solution was used, except that the name of the agar used is not mentioned. Please can anyone help me out with this agar to use. I am trying to carryout an ROS production test on the worms. Thank you in anticipation. ---
**Author:** ![system](https://community.alliancegenome.org/uploads/default/original/1X/d0a649bda118ecbfde158d2e012a0648410be778.png) [@system](https://community.alliancegenome.org/u/system)\ **Post date:** [September 15, 2014, 11:16am UTC](https://community.alliancegenome.org/t/for-2-agar-pads/4984/2 "2014-09-15T11:16:31Z")
just to be clear, are you perhaps reading different sections/protocols where people describe 5% agar pads and 2% agarose pads? In any case, the agar we use works fine for pads (Agar Agar Kobe I) ---
**Author:** ![system](https://community.alliancegenome.org/uploads/default/original/1X/d0a649bda118ecbfde158d2e012a0648410be778.png) [@system](https://community.alliancegenome.org/u/system)\ **Post date:** [September 18, 2014, 7:57am UTC](https://community.alliancegenome.org/t/for-2-agar-pads/4984/3 "2014-09-18T07:57:22Z")
OK, Okayforum asked via the Moderator the following: ‘…Yes I plan to used the 2% agar pad made from 2%agar solution method. You mentioned 2% agarose pad. Is that different from the 2% agar pad I plan to use?’ So, to the first question, the answer is chemically; yes. Also the physical attributes of agarose are different, but I guess that’s not what you are getting at right? The point is that there are a swath of different methods. A short summary of which would include: 2% agar (apparently used by someone at Cologne Uni according to this posting: [http://www.researchgate.net/post/How\_does\_one\_mount\_caenorhabditis\_elegans\_on\_a\_slide\_and\_is\_there\_any\_simple\_stain\_to\_observe\_them](http://www.researchgate.net/post/How_does_one_mount_caenorhabditis_elegans_on_a_slide_and_is_there_any_simple_stain_to_observe_them) 3% agar pads - Shakes et al. and others for immunofluorescence. 4% agar pads: [http://www.wormbook.org/chapters/www\_intromethodscellbiology/intromethodscellbiology.html#d0e2311](http://www.wormbook.org/chapters/www_intromethodscellbiology/intromethodscellbiology.html#d0e2311) (Protocol 28). 5% agar pads (same reference, Protocol 1) And of course, 2% agarose pads as previously mentioned. I would try them out and see what suits your set up. Steve ---
**Author:** ![Okayforum](https://community.alliancegenome.org/letter_avatar_proxy/v4/letter/o/f19dbf/32.png) [@Okayforum](https://community.alliancegenome.org/u/Okayforum)\ **Post date:** [September 18, 2014, 2:38pm UTC](https://community.alliancegenome.org/t/for-2-agar-pads/4984/4 "2014-09-18T14:38:48Z")
Thank you so much once again moderator. I was getting my self confused previously but now as regards the ROS production test I intend to carryout on the worms, since I would want the worms to be alive, just static while I take the image capture, I want to use the 5% agar solution method described in [wormbook.org](http://wormbook.org). Just to be completely clear, the agar I am supposed to use for this metho d is Agar Agar Kobe I right? ---
**Author:** ![Snug](https://community.alliancegenome.org/letter_avatar_proxy/v4/letter/s/13edae/32.png) [@Snug](https://community.alliancegenome.org/u/Snug)\ **Post date:** [September 18, 2014, 5:21pm UTC](https://community.alliancegenome.org/t/for-2-agar-pads/4984/5 "2014-09-18T17:21:43Z")
Science is all about experimentation. If you’ve got some agar and agarose already in the lab, why spend more money when you can just do a little experiment yourself and find what condition works best for you. ---
**Author:** ![Okayforum](https://community.alliancegenome.org/letter_avatar_proxy/v4/letter/o/f19dbf/32.png) [@Okayforum](https://community.alliancegenome.org/u/Okayforum)\ **Post date:** [September 18, 2014, 6:59pm UTC](https://community.alliancegenome.org/t/for-2-agar-pads/4984/6 "2014-09-18T18:59:23Z")
I do intend to continue experimenting Snug, but then I need to confirm the type of agar i want to use. ---
**Author:** ![system](https://community.alliancegenome.org/uploads/default/original/1X/d0a649bda118ecbfde158d2e012a0648410be778.png) [@system](https://community.alliancegenome.org/u/system)\ **Post date:** [September 19, 2014, 5:49am UTC](https://community.alliancegenome.org/t/for-2-agar-pads/4984/7 "2014-09-19T05:49:43Z")
just try using (as Snug said) some agar (that’s still in date) out of / on the chemicals cupboard/shelf and see how it goes… remember, how static the worms will be depends on whether you anesthetise them…enough… ---
**Author:** ![Snug](https://community.alliancegenome.org/letter_avatar_proxy/v4/letter/s/13edae/32.png) [@Snug](https://community.alliancegenome.org/u/Snug)\ **Post date:** [September 19, 2014, 1:41pm UTC](https://community.alliancegenome.org/t/for-2-agar-pads/4984/8 "2014-09-19T13:41:24Z")
> [@steveh post:7](#): > > remember, how static the worms will be depends on whether you anesthetise them…enough… be sure to not use sodium azide, too ---
**Author:** ![Okayforum](https://community.alliancegenome.org/letter_avatar_proxy/v4/letter/o/f19dbf/32.png) [@Okayforum](https://community.alliancegenome.org/u/Okayforum)\ **Post date:** [September 25, 2014, 2:49pm UTC](https://community.alliancegenome.org/t/for-2-agar-pads/4984/9 "2014-09-25T14:49:52Z")
Alright, thank you very much for the vital information provided to me.