# Freezing or fixing worms

**URL:** <https://community.alliancegenome.org/t/freezing-or-fixing-worms/7078>\
**Category:** Methods & Reagents\
**Created:** [August 17, 2023, 7:11am UTC](https://community.alliancegenome.org/t/freezing-or-fixing-worms/7078 "2023-08-17T07:11:38Z")\
**Posts on this page:** 2\
**Page:** 1

<div class="post-metadata">

**Author:** ![Sister\_michael](https://community.alliancegenome.org/user_avatar/community.alliancegenome.org/sister_michael/32/337_2.png) [@Sister\_michael](https://community.alliancegenome.org/u/Sister_michael)\
**Post date:** [August 17, 2023, 7:11am UTC](https://community.alliancegenome.org/t/freezing-or-fixing-worms/7078/1 "2023-08-17T07:11:38Z")

</div>

i am trying out protocols for c elegans actin staining. some protocols tell you to snap freeze the worms while some fix the worms with paraformaldehyde before staining. Do the two methods have the same effect on worms?

---

<div class="post-metadata">

**Author:** ![HillelSchwartz](https://community.alliancegenome.org/user_avatar/community.alliancegenome.org/hillelschwartz/32/8_2.png) [@HillelSchwartz](https://community.alliancegenome.org/u/HillelSchwartz)\
**Post date:** [August 17, 2023, 10:58pm UTC](https://community.alliancegenome.org/t/freezing-or-fixing-worms/7078/2 "2023-08-17T22:58:36Z")

</div>

I recommend that you read [the WormBook chapter on Immunohistochemistry](http://www.wormbook.org/chapters/www_immunohistochemistry/immunohistochemistry.html), which includes some discussion of freezing and paraformaldehyde.