i am bit confused about which/how a gene can be selected for qPCR gene expression study.
There are plenty of genes lie within a particular signaling pathway. For instance, you don’t quantify daf-16 activity using qPCR because we observe for localization/delocalization activities instead. qPCR on daf-16 would be meaningless.
While for genes such as mtl-1, sod-3 (stress related), spp-1, lys-8 (antimicrobial-related), we can perform qPCR for query.
anyone may explain how the selection basis is made then? Thank you…