Harvesting bacteria shed by C. elegans

Model Organism: Worms Methods & Reagents
1

In my experiment I will be infecting C. elegans with Salmonella, which are known to be able to survive in the C. elegans gut and to be viable
after being defecated by the worm.

I want to harvest the bacteria immediately after they are defecated. I was wondering if someone has any ideas on how I can achieve this.
I am planning to use a 20 micron sieve test, wash the worms constantly, and then concentrate the bacteria that have been washed off. Any other ideas?

Thanks

Lizeth

2

Look at the recent paper by Kawli and Tan in Nature Immunology. I thought their experiment for measuring live bacteria from the worms guts were quite elegant. I think it could probably be adapted to find what you are looking for.