So Im trying to find a protocol for heat shock to be able to discern HSP-4::GFP expression. What temp and how long? I just need this to screen progeny, and I don’t want to use tunicamycin.
April :wink:

Hey girl friend! You should totally try 10 minutes at 42C, this ended up working for me!

Thanks lady! That seems like great advice from a cool chick.

-for all others, I found it online and did it successfully(super easy to see)…so thanks anyways worm community. :slight_smile: