So Im trying to find a protocol for heat shock to be able to discern HSP-4::GFP expression. What temp and how long? I just need this to screen progeny, and I don’t want to use tunicamycin.
Thanks!
April
Hey girl friend! You should totally try 10 minutes at 42C, this ended up working for me!
Thanks lady! That seems like great advice from a cool chick.
-for all others, I found it online and did it successfully(super easy to see)…so thanks anyways worm community.