# MH27 Staining in Young Adults

**URL:** <https://community.alliancegenome.org/t/mh27-staining-in-young-adults/3871>\
**Category:** Methods & Reagents\
**Created:** [February 22, 2011, 9:31pm UTC](https://community.alliancegenome.org/t/mh27-staining-in-young-adults/3871 "2011-02-22T21:31:18Z")\
**Posts on this page:** 3\
**Page:** 1

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**Author:** ![guitarmanwm](https://community.alliancegenome.org/letter_avatar_proxy/v4/letter/g/aca169/32.png) [@guitarmanwm](https://community.alliancegenome.org/u/guitarmanwm)\
**Post date:** [February 22, 2011, 9:31pm UTC](https://community.alliancegenome.org/t/mh27-staining-in-young-adults/3871/1 "2011-02-22T21:31:18Z")

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I am in need of an optimized protocol for staining young adults with MH27 using freeze crack method and methanol fixation. Does anyone have a protocol that they know will work? If you know of a better method than than freeze crack and methanol fixation it would be ok too.

Thanks,  
Chris

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**Author:** ![Janet\_Duerr](https://community.alliancegenome.org/letter_avatar_proxy/v4/letter/j/5fc32e/32.png) [@Janet\_Duerr](https://community.alliancegenome.org/u/Janet_Duerr)\
**Post date:** [March 3, 2011, 3:06pm UTC](https://community.alliancegenome.org/t/mh27-staining-in-young-adults/3871/2 "2011-03-03T15:06:52Z")

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Chris,

The MH27 antibody works on worms fixed for 20 minutes in 3% formaldehyde (in PBS). You can use freeze-crack to try and open the worms and then apply the formaldehyde solution on the slide. Or you can do rapid repeated freeze-thaws of worms in tubes of 3% formaldehyde - this tends to break open only a subset of the worms (but they don’t float away, as they may with the slides).

Janet

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**Author:** ![guitarmanwm](https://community.alliancegenome.org/letter_avatar_proxy/v4/letter/g/aca169/32.png) [@guitarmanwm](https://community.alliancegenome.org/u/guitarmanwm)\
**Post date:** [March 3, 2011, 8:24pm UTC](https://community.alliancegenome.org/t/mh27-staining-in-young-adults/3871/3 "2011-03-03T20:24:42Z")

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Thanks for the advice. Do you have a paper that references this method?