Although we recently published the below paper addressing this very question in worms, we too have had a problem with the Invitrogen kit being backordered since April. So we switched to the standard Qiagen miniprep kit (with the PB wash included) and have so far been able to reproduce the results described for the Invitrogen kit + PB wash in our paper. We continue to get multiple knock-ins from injecting just 10 worms. This was a pleasant surprise because the Qiagen kit costs us just $1.29 per prep vs. the $5.07 per prep for Invitrogen PureHQ. Hope this helps!
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