Hi All,
Has anyone here worked with mitochondrial UPR markers such as hsp-6 or hsp-60? I wish to induce these with ethidium bromide
Specifically, what concentration of ethidium bromide do I use for treatment (literature suggest 5-125ug/ml is sufficient) and do I dump it onto the plates or add it to the media? Also, is there a specific development stage I should be looking at to see the induction?
Any suggestion/advice would be helpful.
Thanks