The October Papers with Technical Advances are here: https://wiki.flybase.org/wiki/FlyBase:Papers_with_technical_advances#Oct_2025. Don’t forget to flag when you Fast-Track Your Paper if you want your paper with a new technique, reagent, or resource featured next month.
Distinct cellular and molecular mechanisms contribute to the specificity of the two Drosophila melanogaster chitin synthases in chitin deposition. (PLoS Genet.)
We have generated new transgenes carrying the full length Chs2 tagged with GFP. We have also generated transgenes of chimeras Chs2 and Kkv proteins. We have generated an antibody against Chs2
https://pubmed.ncbi.nlm.nih.gov/40920808
Protocol to quantify compound retention in Drosophila melanogaster for the pharmacokinetic study of orally administered compounds. (STAR Protoc)
This paper includes a description of a new method to research the bioavailability of the orally administered compounds in Drosophila melanogaster, and therefore aids in the metabolomics and nutrition research
https://pubmed.ncbi.nlm.nih.gov/40711873
Repressive S-adenosylmethionine biosynthesis status inhibits transcription of HeT-A retrotransposon in the germline of Drosophila. (J. Biochem., Tokyo)
anti-Sam-S polyclonal antibody
https://pubmed.ncbi.nlm.nih.gov/40599133
Protocol to measure temporal consumption and feeding choices of Drosophila adults using capillaries. (STAR Protoc)
Procedures and device to measure feeding from liquids in Drosophila adults
https://pubmed.ncbi.nlm.nih.gov/40638379
Replication competition drives the selective mtDNA inheritance in Drosophila ovary. (Cell Rep.)
We present a highly specific and efficient in situ imaging method, termed “CAR” (Circular DNA Amplification at Restriction enzyme sites), capable of visualizing mtDNA variants that differ by only a few nucleotides at single-molecule resolution in Drosophila ovaries.
https://pubmed.ncbi.nlm.nih.gov/40911417
Protocol for a two-clone system to study signaling interactions among neuronal cells in a pre-clinical Alzheimer’s disease model. (STAR Protoc)
Genetic mosaic approach
https://pubmed.ncbi.nlm.nih.gov/40782346
OligoY pipeline for full Y chromosome painting. (Brief. Bioinform.)
We developed a pipeline that allows designing FISH Oligopaint probes for highly repetitive regions of Y chromosome. We tested it on Drosophila melanogaster’s Y chromosome. Our FISH assays showed no off-target staining and many additional regions of the Y chromosome to be stained.
https://pubmed.ncbi.nlm.nih.gov/41025923
Arthropod Pax Gene Evolution: A Role for Vanessa Cardui Twin of Eyeless in Eye Development. (Mol. Biol. Evol.)
Expression analysis by in situ hybridization of members of the Pax gene family in a new butterfly model system, Vanessa carduii, the painted lady butterfly. Embryonic RNAi to test gene function in Vanessa.
https://pubmed.ncbi.nlm.nih.gov/40966593
Precise measurement of motor neuron dysfunction in Drosophila ALS model via climbing assay and leg imaging. (Methods Cell Biol.)
Development and introducing of a novel user-friendly and quick method for measuring locomotion activity in Drosophila melanogaster (fruit fly), which is applicable in a wide spectrum of diseases related to motor neuron dysfunction, such as ALS.
https://pubmed.ncbi.nlm.nih.gov/40930692
Mitochondrial Complex I and ROS control neuromuscular function through opposing pre- and postsynaptic mechanisms. (PLoS Biol.)
We describe many ways to impair Mitochondrial Complex I function in Drosophila tissues, both genetically (RNAi and mutant) and pharmacologically (rotenone).
https://pubmed.ncbi.nlm.nih.gov/40982549
What the fruit fly can tell us about autosomal recessive primary microcephaly. (Fly)
We generated a deep learning AI model for automated segmentation of the adult fly brain from microcomputed tomography imaging. This model is freely available and can be adapted to individual user needs.
https://pubmed.ncbi.nlm.nih.gov/41074654
Hatching-Box: Automated in situ monitoring of Drosophila melanogaster development in standard rearing vials. (PLoS ONE)
New resource to measure eclosion experiments in vials