Hi! Does anyone know where I can get the original sequence end-reads for the WRM0628cF10 fosmid?? I’m carrying out a RE digest and I wanted to have an idea of the RE to use as I don’t want to cleave within the pcc1Fos backbone.
Thanks
Hi there,
If you go to the following site, you can search for your clone, and if you click on the clone link, it will give you the two reads:
http://elegans.bcgsc.ca/perl/fosmid/CloneSearch
If this link works, it’s a direct link to the sequence:
http://elegans.bcgsc.ca/perl/fosmid/endreads?clone=WRM0628cF10;