outcross

Model Organism: Worms Scientific Discussion
1

Hi, everyone
I would like to get the 8X outcrossed worms. so, how many times should I put the worms of interest(hermaphrodites) to mate with N2(males)? Do I get the 2X outcrossed worms once I got the F2 homozygote? I mean, should I repeat the process 7 times or just 3 times? I hope you understand what I want to express. Thank you in advance

2

Hi,

just to be clear…are you asking about 8x backcrossed worms?

http://www.wormatlas.org/glossary/bglossary.htm#backcross

rather than 8x outcrossed worms??

http://www.wormatlas.org/glossary/oglossary.htm#outcross

Steve

3

Thanks Steven, the information is very useful for me. I compeletely thought the two terms stand for the same meaning. Now I am not confused. What I want to know is about “backcross”. That is,how many times should I execute the process(mating the worm strain of interest with N2 males) if I would like to get the 8X backcrossed worms?

4

Hi,

take a look at;

http://www.wormbook.org/chapters/www_introreversegenetics/introreversegenetics.html#d0e928

Go to section 7.4.2.2. Backcrossing strategy.

This outlines how you set up (and count) backcrosses.

Hope this helps getting you started…it takes a while!

Steve

5

Thanks for your kindly help.