Has anyone tried to culture C. elegans using pasteurized OP50? Does anyone know how the pasteurized food affect behaviors and lifespan?
Is there a strict definition of “pasteurized”? When I look it up, I just find claims that it means to kill (or even just to mostly kill) bacteria, usually either using heat or radiation.
There is an extensive (if possibly somewhat scattered) literature using <a href=https://www.google.com/search?q=%22uv-killed%22+bacteria+elegans">uv-killed and heat-killed bacteria as a food source for C. elegans.
Thank you.
I have searched “heat-killed”, then I at least found these three papers showing a survival curve of C. elegans cultured by pasteurized OP50.
Ann N Y Acad Sci. 2007 Apr;1100:530-42.
Evidence for a trade-off between survival and fitness caused by resveratrol treatment of Caenorhabditis elegans.
Gruber J1, Tang SY, Halliwell B.
Mech Ageing Dev. 2009 Aug;130(8):477-86. doi: 10.1016/j.mad.2009.05.005. Epub 2009 Jun 6.
Catechin induced longevity in C. elegans: from key regulator genes to disposable soma.
Saul N1, Pietsch K, Menzel R, Stürzenbaum SR, Steinberg CE.
Infect Immun. 2002 Aug;70(8):4705-7.
Diverse bacteria are pathogens of Caenorhabditis elegans.
Couillault C1, Ewbank JJ.