Positive controls for PCR

Hi,

I’ve had problems with my PCR - trying to sequence 18S area from different kinds of nematodes - and now I’m trying to come up with positive controls to help troubleshooting. My supervisor suggested using actin gene for positive control. I’ve tried to look around, but haven’t found any universal primers for all nematodes for any of the actin genes. Is anyone using successfully primers for actin?

Tuomas Aivelo,
from U. of Helsinki