We are working with an endoplasmic reticulum resident protein and we have made a YFP fusion of this protein containing both the ER signal peptide and the KDEL retention signal.
We want to confirm the ER lumen localization by using any well established ER marker with RFP or mCherry
I will very much appreciate if anyone can let me know of such a RFP/mCherry marker
Involvement of the Actin Cytoskeleton and Homotypic Membrane Fusion in ER Dynamics in Caenorhabditis elegans
Dmitry Poteryaev * , Jayne M. Squirrell , Jay M. Campbell , John G. White , and Anne Spang *
Friedrich Miescher Laboratorium, Max Planck Gesellschaft, D-72076 Tübingen, Germany; Laboratory of Molecular Biology, University of Wisconsin, Madison, WI 53706
Submitted August 22, 2004; Revised February 4, 2005; Accepted February 7, 2005
Monitoring Editor: Reid Gilmore