RFP construct

Model Organism: Worms Methods & Reagents
1

Does anyone know of an RFP construct that expresses well in worms? I am looking for an RFP that has been modified like GFP from the Fire
vectors to create a pan-neuronal RFP-expressing worm and have no experience in this field.
Any help would be greatly appreciated. Thank you.

2

Does it have to be RFP, or is mCherry acceptable? If so, pGH8 in Erik Jorgensen’s MosSCI kit might work for you. I have subcloned various neuronal promoters into this vector and also used the vector as a template for PCR promoter fusions to mCherry, both with great success. The mCherry in this vector expresses very nicely in worms. You can obtain the vector through Addgene, I believe.

Hope this helps,

Allen Andrews

3

Thanks, Allen. I’m just looking for a red fluorescent protein, doesn’t have to be RFP, so I’ll look into your suggestions.

4

We have some good unc-119::mCherry strains made from an unc-119::mCherry reporter. These express nicely in neurons. Contact me if interested (mmaduro@ucr.edu).