RNA polymerase II ChIP

Does any one have experiences with RNA polymerase II ChIP in worms?

I know people got dpy-27 and several other proteins to work. But I did not notice whether people reported RNA polymerase II.

thanks so much.


RNA pol II antibody : Santa Cruz, sc-899

do you have a protocol or a reference for that?

thanks so much.

I tried 8WG16, CTD4H8, ARNA-3. I don’t know whether it is the antibody problem or my techniques.

We’ve done ChIP-chip with 8WG16 and it works reliably well using the Ercan et al paper protocol. The data is currently unpublished though you should be able to view it soon on the modENCODE site (modencode.org).