run-on

Model Organism: Worms Methods & Reagents
1

Hi,

Does any one have a protocol to do nuclear run-on assay in worms?

Thanks very much.

2

I think these 2 papers would help you get started ;D

http://www.jbc.org/cgi/reprint/254/18/9050.pdf

http://www.genetics.org/cgi/reprint/126/4/889.pdf

Hope this helps,

Luv

3

Thanks very much.

I sort of get the nuclear run on to work now with a protocol adapted from Jis Lis’s lab.