Sudan Black

Model Organism: Worms Scientific Discussion
1

Dear all,

For staining worm lipid droplets generally what percentage of Sudan black solution is used?
i.e. how much gram of Sudan Black powder is dissolved in 100ml of 70%Ethanol solution?

Thanks…

2

Typically, it’s a saturated solution (i.e., keep adding/mixing until a precipitate remains). Filter with syringe and 0.45 micron filter before use.