Hello,
I would like to test the influence of different stresses on the number of offspring of my mutants.
In the literature the best studied stresses are radical-, osmotic- and heat-stresses. I am especially looking for stresses where the downstream endogenous stress response has been studied thoroughly or even has been profiled transcriptome-wide as for example in Shin et al. (Jones Lab) 2011 (http://www.biomedcentral.com/content/pdf/1756-0500-4-34.pdf).
Some preliminary experiments with osmotic stress and heat resulted in sterility of all worms.
I wonder if anyone has experience with this topic or could point out some hallmark papers to look at.
Thanks in advance,
Marlon
The transcriptional response to osmotic stress, as well as several osmotic stress resistant mutants, was profiled in this paper:
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0009010
Previous work also found that osmotic stress induced sterility. Reduced glycerol production enhanced osmotic stress-induced sterility (Lamitina et al, 2006, "Genome-wide RNAi screening identifies protein damage as a regulator of osmoprotective gene expression, PNAS).
Heat shock transcriptional data was previously profiled in:
GuhaThakurta et al, 2002, “Identification of a novel cis-regulatory element involved in the heat shock response in Caenorhabditis elegans using microarray gene expression and computational methods.” Genome Research
and
McCarroll SA, Murphy CT, Zou S, Pletcher SD et al. Comparing genomic expression patterns across species identifies shared transcriptional profile in aging. Nat Genet 2004 Feb;36(2):197-204.
Good luck!
Thanks!