2 Postdoctoral opportunities in **Prof Anna Philpott’s laboratory, Cambridge Stem Cell Institute**

Background
Lineage fidelity and infidelity in stem cells and development

Mechanisms that lead to the establishment and maintenance of cell identity are paramount for organismal health. They also underpin successful cellular reprogramming for disease modelling and cell replacement therapies. Alongside experimental lab work using embryonic stem cells, cancer cells and Xenopus frog embryos, the Philpott lab, in collaboration with the Simons lab, makes use of analytical and computational approaches to study mechanisms of lineage fidelity and transcriptional reprogramming of cell fate during development and in disease. Current interests include the roles played by the epigenome and cofactors in regulating lineage transcription factor-mediated establishment and stabilisation of cell fate.

There are currently two post-doctoral opportunities available in the Philpott lab:

Post-doctoral Research Associate - Investigating lineage fidelity during development

This project will use single cell transcriptomic and multi-omic analysis to determine the cellular response to transcription factor reprogramming and will focus on the response of cells using the in vivo Xenopus embryo system (full training given), while aligning closely with related work on ES cells in the lab. This project may use many techniques including genome-wide analysis of gene expression in single and multiple cells, chromatin binding and accessibility studies, CRISPR genome editing as well as classical embryology and biochemistry.

And

Computational Biology Research Associate - Single-cell and multi-omics approaches to mechanisms of cellular programming and reprogramming in development

With experience in the development of statistical, computational and bioinformatics methods, this RA will have a key position in analysing single-cell and bulk genome-wide datasets across a range of projects and experimental model systems. As well as performing computational genomics analyses, this RA will also make use of functional data to develop mechanistic insights using statistical modelling-based methods and/or computational approaches, working closely with experimental colleagues to transform sequencing data into biologically meaningful information. Reflecting this central role in data analysis and integration, the RA’s contributions will be acknowledged accordingly.

Both opportunities are fixed term (to April 2026 in the first instance) and are based in the Cambridge Stem Cell Institute in the University of Cambridge. For more details of the positions and links to further particulars, please go to:

Post-doctoral Research Associate - Investigating lineage fidelity during development (Fixed Term) - Job Opportunities - University of Cambridge

Computational Biology Research Associate - Single-cell and multi-omics approaches to mechanisms of cellular programming and reprogramming in development (Fixed Term) - Job Opportunities - University of Cambridge

Informal enquiries to ap113@cam.ac.uk welcomed.

Lab website: Professor Anna Philpott | Wellcome-MRC Cambridge Stem Cell Institute

Deadline for applications: 4th April 2023