I’m just getting started with C. elegans and while looking over publications and posts I notice that some people use 8P (peptone rich) plates for growing NA22 for large volumes of c. elegans. Other labs use NGM plates with NA22 and C. elegans. Can anyone tell me the basis for this decision? I assume the nutrients used to feed the e coli affect the nutrients taken up by the C. elegans but how do you know which agar base you need to start with?
Thanks