THE FAY LAB ANNOUNCES A HANDY NEW CRISPR DESIGN TOOL – CRISPRcruncher
Takes any coding sequence and displays a comprehensive list of minimal substitutions that produce new restriction endonuclease sites while maintaining the original peptide sequence
https://crisprcruncher.io/
CRISPRcruncher is perfect for designing repair templates with new RE sites
Scans sequences 15–99 bp long
Enzyme library contains >650 REs
Coded entirely by 19-yo Sam Fay, home on “college-covid leave”!!!
Mentorship provided by Vikram Chhatre, Wyoming INBRE Bioinformatics Core
Using a 99-bp coding sequence, CRISPRcruncher will typically identify >100 new sites for 6+ cutters and >500 new sites for 4+ cutters. Not too shabby!
Find out what you’ve been missing, optimize repair templates, increase success rates, use saved time to scroll Twitter feed!
Disclaimer: CRISPRcruncher is probably useless if you’ve memorized the genetic code along with every restriction enzyme consensus site and are the genetic equivalent of a chess Grandmaster.
If this is NOT the case, the tool may prove quite useful.