A third GFP to use with myo-2 and myo-3

Model Organism: Worms Methods & Reagents
1

I’m looking for a third GFP marker that can be used with and distinguished from myo-2 and myo-3. I tried sur-5, but the signal makes it hard to detect whether myo-2 is there or not. It is for marking a transgene, so I need a DNA construct, not a line with an integrant. I have the Fire Lab kit and am hoping that some other marker in the kit will work. Any suggestions?

Thanks,

Tim Kroft

2

Hi Tim,

If you are looking for a bright and reliable marker that is not expressed in muscle, consider unc-122::GFP (unc-122 is also known as ofm-1) which drives expression beautifully in the coelomocytes and nowhere else (in adults).

It’s available at Addgene thanks to Piali Sengupta’s lab, in both rfp and gfp versions; here’s the link to the GFP:

http://www.addgene.org/pgvec1?f=c&cmd=findpl&identifier=8937&attag=fh

Hope this helps!

3

Thanks for the tip.

I need a marker that is visible in L4s so I can pick worms during crosses. I’ve seen cc::GFP before but can’t remember if it comes on before the adult stage. Do you remember?

-Tim

4

Hi Tim,

unc-122/ofm-1::GFP expression can be seen in embryos. In my impatience, I often check injections 24hrs after finishing and can spot “green eggs.” It is very easily seen in L4s too.

If you’d like any more details, you can also feel free to contact me directly.

Matt

5

Thanks, Matt. That’s just what I’m looking for. I’ll order it.

-Tim