I have visited Aceprimer, but it does not work for me.
I have sent an error message via email address shown after error message, but no response.
I want to genotype a KO allele created by CRISPR by PCR.
I made a KO for transposon gene, which has multiple similar sequence in the genome.
For genotyping, I need an algorithm to give a set of primers from the position of the genome.
Does anyone know a good website for primer design.
Also, does anyone know the status of Aceprimer? I’d appreciate if anyone would let me know about Aceprimer.