I have visited Aceprimer, but it does not work for me.
I have sent an error message via email address shown after error message, but no response.

I want to genotype a KO allele created by CRISPR by PCR.
I made a KO for transposon gene, which has multiple similar sequence in the genome.
For genotyping, I need an algorithm to give a set of primers from the position of the genome.
Does anyone know a good website for primer design.
Also, does anyone know the status of Aceprimer? I’d appreciate if anyone would let me know about Aceprimer.


The interface is a little clunky but primers suggested by Primer3 have always worked for me. At least that’s true at the standard settings, for moderately sized products; if I desperately need exactly a particular primer position or I’m trying to amplify a very large product, and so fiddle with the settings to get a primer I like, it’s less certain to work.