hi everyone, did someone do western blot for c.elegans, and what kind of actin antibody ? thanks.
We use a mouse monoclonal antibody (clone C4) from MP Biomedicals at 1:5000 for C. elegans Westerns.
Thank you ,dspoole .
Best and most economical solution is mouse monoclonal anti-chicken gizzard actin from Developmental Studies Hybridoma Bank (DSHB)
http://dshb.biology.uiowa.edu/
The antibody number is JLA20. We use for C. elegans Westerns at 1:2000 & get extremely clean blots. Price is unbeatable at $40/0.1 mL.
This also helps support a non-commercial national resource.