actin antibody for c.elegans

lieggplant · February 4, 2009, 6:01am

hi everyone, did someone do western blot for c.elegans, and what kind of actin antibody ? thanks.

dspoole · February 4, 2009, 6:38pm

We use a mouse monoclonal antibody (clone C4) from MP Biomedicals at 1:5000 for C. elegans Westerns.

lieggplant · February 5, 2009, 12:42pm

Thank you ，dspoole .

ljacobson1 · February 10, 2009, 3:47pm

Best and most economical solution is mouse monoclonal anti-chicken gizzard actin from Developmental Studies Hybridoma Bank (DSHB)
http://dshb.biology.uiowa.edu/
The antibody number is JLA20. We use for C. elegans Westerns at 1:2000 & get extremely clean blots. Price is unbeatable at $40/0.1 mL.

This also helps support a non-commercial national resource.