Dear Worm breeders,
We have generated a large number of transgenic worms in an unc-119 background using microparticle bombardment. We need large numbers of these transgenics and have been growing them in liquid culture to reach the density required. A few months ago, these strains were growing really well with a quick generation time. More recently, the same strains have become extremely slow growing and have not been reaching the densities needed.
We have tried starting fresh cultures from frozen stocks, making fresh culture reagents etc, but nothing as improved the growth of these strains. The same strains have also not grown well when we have put them on solid media.
Has anyone had any experience with the long-term liquid culture of transgenics?? Is it perhaps the time of year, as it is now winter in the Southern hemisphere!
Any help is appreciated.
Thanks in advance.
Julie-Anne