Does anyone have suggestions for how to reliably aliquot approximately the same number of eggs to multiple plates following bleaching of adults? I’m trying to compare multiple RNAi clones simultaneously and want to get about the same number of growing worms per RNAi plate.
Even with frequent mixing, the eggs in M9 distribute pretty non-homogeneously and I end up getting a lot of variation in eggs per plate after aliquoting. I’m wondering if anyone has resuspended eggs in something more viscous to prevent settling?
Any advice would be greatly appreciated.