ALM and PLM specific rescues

Model Organism: Worms Scientific Discussion
1

Hi there,

I would like to do ALM and PLM specific rescues (the mec-4 promoter should be suitable for this right?). Does anybody know if there is also a way to discriminate between the two?

Regards,
Ellen

2

use ZDIS4 which contains a transgene mec-4::gfp which helps in visualisation of mechanosensory neurons…
So under the fluorescent microscope you can easily distinguish between ALM & PLM.

3

Thx, but what I actually want to accomplish is expression of a certain gene under an ALM or PLM specific promoter instead of a common promoter such as Pmec-4…