We use ethanol burners or gas burners to sterilize picks and our spatulas for chuncking worms- but I am wondering if anyone has a good, possibly non open flame alternative? I’m trying to appease the safety officers! I’m looking for some other set up, or confirmation that ethanol burners are the absolute norm in worm labs.
I think Sydney Brenner’s original paper describes picking worms with sterile toothpicks. Seems reasonable if you have a steady hand. But please tell your safety officers that etoh lamps are currently the norm. Pt wire picks are much more convenient. I also like chunking with a thick bacterial loop rather than with a spatula since you can skip dipping it in ethanol…