Can anyone suggest an anti-GFP antibody that works well for Western Blots with C. elegans lysates? I am trying to detect a GFP fusion protein that is about 70kD in size. I am currently using the chicken anti-GFP IgY antibody from Millipore, but it seems to bind non-specifically to something in the worm lysate that is about 70kD, which is making my life difficult.
I don’t have a good one for you, but I have used Santa Cruz Biotechnology sc-9996 and I would NOT suggest that, as it gives a lot of background.
Good luck.
I have successfully used a rabbit anti-GFP for westerns. Invitrogen A-6455. It recognizes sur-5::GFP from boiled worm lysates great.
We use the Roche Antibody which is a mixture of two clones (Cat. No. 11814460001 ). Works very well for Westerns and also for IPs.
Thank you all for your suggestions. Knowing what not to get can be as important as knowing what works.