Hello Worm Community,
I was wondering if anyone has experience with an anti-RFP antibody that works effectively in worms for immunostaining. Any recommendations or insights would be greatly appreciated!
Thank you in advance!
Depending on your RFP, the RFP antibody (5F8) from Chromotek (now Proteintek) works quite well in staining. It doesn’t work with tagRFP though
(even though they say it is for IF and ELISA, I have used it for western blot as well but not IP. If you are interested in IP, the RFP Trap from the same company is good)
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Good luck