I want to know which antibiotics can be added in NGM to prevent contamination, but I didn’t find the answer in any paper.
Could someone help me? Thank you！
Typically I don’t think most people use antibiotics; the standard food strains (OP50, HB101) aren’t antibiotic-resistant. Often Ampicillin (or Carbenicillin) and sometimes Tetracycline are added to the NGM for RNAi feeding experiments, but these are used to maintain markers in the feeding strain rather than to avoid contamination.
If you have a problem with contamination, it’s usually recommended to make sure that you are seeding plates with bacteria grown from a single streaked colony and that you bleach your strains as needed to remove bacterial or yeast contamination. Mold contamination can usually be avoided by keeping your work and storage areas clean and sealing old plates in parafilm, and mold can often be removed without bleaching the animals if you transfer the animals to a clean plate, mark the spot to which they are transferred, and bleach that spot after the animals have left it.
A Wormbook chapter gives guidance on cleaning strains and also cites a reference (Caldicott et al) for adding drugs to NGM.
The main exception I can think if is that some people supplement media with Nystatin to impede fungal growth (specifically in microparticle bombardment), and a couple of cases (of many more, probably) in which people use antibiotics but are adding bacterial food sources grown separately, at fixed concentrations.
:)Thank you very much!
Actually OP50 is streptomycin resistant. You can add streptomycin in NGM. For anti fungus, you can add Nystatine.