Does anyone every create reporter with GFP and mCherry under the same promoter and see different results?
Please share.
What have you seen that’s different? Since GFP is much brighter than mCherry, I could imagine that if you were looking at the timing of promoter activation you might see GFP signal before you saw mCherry signal.
we’ve seen m-cherry tagged proteins assemble into filaments in muscle cells. gfp-tagged versions didn’t do this.
I actually see dsRED (just realized it’s dsRed not mCherry - sorry) much brighter than GFP, and is detected in more cells.
This is the opposite observation from everyone else’s it seems, so I’m just trying to understand why.