The FlyBase Papers with Technical Advances page has updated with papers submitted in April.
If you’d like to be featured in an upcoming update, please flag your paper with a new resource, technique, or reagent when you Fast-Track Your Paper.
Strain variation identifies a neural substrate for behavioral evolution in Drosophila. (Curr. Biol.)
Closed loop optogenetic system for replicating inhibitory pheromonal feedback during courtship
https://pubmed.ncbi.nlm.nih.gov/41763206
Frazzled/DCC directs spatial progenitor integration ensuring steady-state intestinal turnover. (Nat. Commun.)
new Hamelin assay for remote controlled testing of chemoattraction of UAS-expressed ligands and migration enable by the use of dual expression systems (Gal4/UAS and LexA-Aop).
https://pubmed.ncbi.nlm.nih.gov/41832280
Asymmetry and the cytoskeleton: Mechanisms of asymmetric neural stem cell division in Drosophila melanogaster. (Curr. Topics Dev. Biol.)
Bioinformatics tool to facilitate the planning of CRISPR/Cas9 genome engineering experiments
https://pubmed.ncbi.nlm.nih.gov/41856738
Noc1 downregulation induces nucleolar stress and upregulates p53 isoforms, with a robust increase of the truncated p53E isoform in Drosophila wing discs. (G3 (Bethesda))
new sets of primers for p53 isoforms in D melanogaster
https://pubmed.ncbi.nlm.nih.gov/41533679
Nup107 is a crucial regulator of torso-mediated metamorphic transition in Drosophila melanogaster. (eLife)
a pair Nup107 gRNA lines, polyclonal Nup107 antibody, Nup107 fragment cloned in bacterial expression plasmid
https://pubmed.ncbi.nlm.nih.gov/41805630
Protocol for cell-specific RNA expression profiling and lipidomics analyses of Drosophila melanogaster intestinal progenitor cells. (STAR Protoc)
Isolation of intestinal stem cells for integrated lipidomics and transcriptomics
https://pubmed.ncbi.nlm.nih.gov/41206871
Systematic generation of Drosophila Wnt transgenes enables the characterization of canonical Wnt signaling. (Fly)
UAS-Wnt transgenes untagged (better function)
https://pubmed.ncbi.nlm.nih.gov/41667411
Somatic gene repression ensures physical segregation of germline and soma in Drosophila embryos. (EMBO Rep.)
We have done bulk and single cell RNA-seq analyses using pole cells isolated from wild-type (y w) and embryos produced from females over-expressing importin alpha2 in pgc (polar granule component) mutation. RNA-seq data were deposited in the DNA Data Bank of Japan (DDBJ).
https://pubmed.ncbi.nlm.nih.gov/41663761
Protocol for the isolation and immunoprecipitation of cell surface proteins from Drosophila melanogaster pupae. (STAR Protoc)
Protocol for the isolation and immunoprecipitation of cell surface proteins from Drosophila melanogaster pupae
https://pubmed.ncbi.nlm.nih.gov/41581153
Visual recognition of the anteroposterior female body axis drives spatial elements of male courtship in Drosophila. (G3 (Bethesda))
Study uses a new machine assisted approach for the spatial analysis of male courtship behavior.
https://pubmed.ncbi.nlm.nih.gov/41699762
Phage display-mediated immuno-PCR to detect low-abundance secreted proteins in Drosophila. (Proc. Natl. Acad. Sci. U.S.A.)
Phage-displaying nanobody, sensitive measurement of fly secreted proteins can be done. The nanobodies can be generated through nanobody screening, or NbVHH05 and Nb127D01 nanobodies can be used to detect endogenous secreted proteins tagged with tandem NanoTags (VHH05 and 127D01).
https://pubmed.ncbi.nlm.nih.gov/41941621
The Drosophila maternal-effect gene abnormal oocyte (ao) does not repress histone gene expression. (Genetics)
New ao reagents: a CRISPR/Cas9-mediated knockout of the ao allele; ao rescue transgene on 3rd chromosme; an epitope-tagged ao allele
https://pubmed.ncbi.nlm.nih.gov/41642668
A Cluster of Three snoRNAs Including Jouvence Required in the Gut Determines Lifespan and Confers Neuroprotection Through Metabolic Parameters. (Aging Cell)
- Lipidomic analysis of the fly, including a precise description of the different sterol. 2) Nile Red staining (brain and pericerebral fat body) allowing to evaluate the nature of the brain vacuoles 3) Bodipy C11-581/591 staining to determine the lipid peroxydation.
https://pubmed.ncbi.nlm.nih.gov/41913725
Identification of optimal fluorophores for use in the Drosophila embryo. (Mol. Biol. Cell)
A set of 17 fly lines, each stably expressing, from the same genomic locus, a green or red fluorescent protein fused to histone H2Av under control of histone H2Av’s promoter and, the constructs/plasmids used for the generation of the fly lines.
https://pubmed.ncbi.nlm.nih.gov/41705958
Glucose is dynamically regulated by time of day in humans and Drosophila. (PLoS Biol.)
We developed a customized analytical platform to identify physiological metabolite homeostatic rhythms in Drosophila via isotope tracing of glucose.