The FlyBase Papers with Technical Advances page has updated with papers submitted in August.
If you’d like to be featured in an upcoming update, please flag your paper with a new resource, technique, or reagent when you Fast-Track Your Paper.
Highly frequent undesired insertional mutagenesis during Drosophila genome editing. (PLoS Genet.)
We show conventional CRISPR/Cas-HDR genome editing frequently (~50% of editing events) causes unintended genome integration of the HDR template vector across loci and editing designs. A new vector, pVID, enables these HDR template integration events to be detected and excluded.
https://pubmed.ncbi.nlm.nih.gov/42424384
Long-Stokes-Shift mScarlet3 as a Structural Marker for Two-Photon Imaging. (MicroPubl Biol)
Tested lssmScarlet3 as an imaging tool in Drosophila brain. (Used jGCaMP8m in the paper.)